Analysis of Protein Fractions of Cerebrospinal Fluid by Means of Disc-electrophoresis (Polyacrylamide-Gel Electrophoresis)
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چکیده
منابع مشابه
Polyacrylamide gel electrophoresis of spinal-fluid proteins.
Twenty-three control samples of CSF were analyzed by vertical column electrophoresis on acrylamide gel. Quantitation results using a microdensitometer are presented. The normal protein pattern shows 15 constant bands which coincide in mobility and in many cases in staining pattern with serum proteins. The haptoglobin peaks, except for the band of the 1-1 type and the leading band of the 2-1 typ...
متن کاملAnalysis of "native" Cerebrospinal Fluid by the Polyacrylamide Disc Electrophoresis Technique.
The polyacrylamide disc electrophoresis technique has been applied to the fractionation of ;native' cerebrospinal fluid. Evidence is presented to show that there is an alteration in the protein profile if the fluid is subjected to a concentration stage. A minimum of 22 distinct protein zones have been detected in fluids from patients without structural changes in the nervous system. The method ...
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Cerebrospinal fluid (CSF) proteins with molecular masses of < 150,000 Da were identified by immunoblotting after two kinds of nonreducing sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). With PAGE 1 (17-27% gradient gel), CSF proteins were clearly separated into seven to nine bands with molecular masses of 3000-67,000 Da; seven bands were identified as beta 2-microglobulin,...
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A simple, rapid method is described for separating the serum lipoproteins into clear, discrete and reproducible bands by polyacrylamide disc gel electrophoresis. The sample is prestained with Sudan Black B in a sample gel and resolved by electrophoresis in a discontinuous pH system consisting of a sample gel, concentrating gel and separating gel. 30 young healthy males and 20 young healthy fema...
متن کاملPolyacrylamide Gel Electrophoresis
Polyacrylamide gels are typically formed by polymerization of the monomer acrylamide crosslinked to the co-monomer, N,N’-methylenebis-acrylamide, commonly called BIS. This process is a freeradical polymerization that requires an initiator, usually ammonium persulfate, and a catalyst like N,N,N’,N’-tetraethylmethylenediamine (TEMED). A distinct advantage of polyacrylamide gel systems is that by ...
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ژورنال
عنوان ژورنال: SEIBUTSU BUTSURI KAGAKU
سال: 1966
ISSN: 0031-9082,1349-9785
DOI: 10.2198/sbk.11.327